Guidelines for Submission of Samples

General recommendations:

Samples suspected of zoonotic diseases must be clearly indicated.

Highly contagious material needs prior notification (e.g. rabies) to CVRL.

Each sample demands for an individual container.

All correspondance must be in English so as to comply with international rules and regulations.

Monkeys are only tested when samples are kept in formalin. No necropsies will be performed.

No samples especially carcass are accepted when the requisition forms are not filled out properly. We have 6 requisition forms, use the right ones. Please give us a proper history about the animals involved including:

  1. How many animals are sick, how many died?
  2. Acute or chronic outbreak?
  3. Clinical signs?
  4. Vaccination, treatment?

Necropsy:

  1. Dead animals (as soon as possible after death)
  2. Moribund animals
  3. Complete anamnesis
  4. Precise labelling
  5. Packing/container/swabs - preferably to be collected from CVRL (free of charge)

Bacteriology/Mycology:

 1. Faecal sampleSealed faecal container
 2.  Cloacal swabAvian - virus medium
 3.  Faecal swab, mammalsBakteriette
 4.  Crop swabBakteriette
 5.  Tracheal swabBakteriette
 6.  Eye swabBakteriette
 7.  Wound swabBakteriette
 8.  Abscess swabBakteriette
 9.  Sinus swabBakteriette
10. Genital swabBakteriette
11. Skin (crust) scrapingPetri dish (sealed)
12. Chlamydial swabSpecial medium
13. Fluid (urine)Plastic tube
14. OrgansSeparated, cooled, Petri dish or plastic tube (sealed)
15. CEM - AmiesCharcoal medium
16. Whole bloodOxoid SIGNAL blood culture system medium

Parasitology:

1. Faecal sampleSealed container
2. Whole blood sampleEDTA vacutainer, purple stopper
3. Skin scrapingPlastic container or sealed petri dish with scalpel blade
4. ParasitesPlastic container, preferably in Formalin or Alcohol
5. ExudatesPlastic tubes

Virology:

1. Cloacal swabVirus transport medium
2. Tracheal swabVirus transport medium
3. Eye swabVirus transport medium
4. CrustsSealed petri dish or plastic tubes
5. OrgansFrozen, plastic tubes
6. Whole bloodIn EDTA-vacutainer from Febrile animal, 3-5 tubes
7. Fluid from blistersSealed plastic tubes

Molecularbiology:

1. Whole blood (unclotted blood)EDTA vacutainer (4 ml), yellow stopper or vacutainer ACD, Solution B
2. Feathers/hairWith roots in plastic bags sealed
3. Filter paper stripsISOCODE filter strips
4. Any biopsyPlastic tube

Tubes available at CVRL for Blood:

1. Whole blood (unclotted blood)EDTA vacutainer (4 ml), yellow stopper or vacutainer ACD, Solution B
2. Feathers/hairWith roots in plastic bags sealed
3. Filter paper stripsISOCODE filter strips
4. Any biopsyPlastic tube

Haematology/Biochemistry:

1. Whole blood (unclotted blood)EDTA vacutainer (4 ml), purple stopper
2. Serum (clotted blood)Fresh, vacutainer, amount; 5 - 8 ml, brown stopper
3. Blood smearsDried slide, wrapped in aluminium foil
4. Whole blood-avianK2 EDTA-Microtainer
5. Coagulation studiesSodium citrate, Monoject

Analytical Chemistry:

1. Unclotted bloodEDTA vacutainer (4 ml), purple stopper
2. Clotted bloodFresh, vacutainer, amount; 5 - 8 ml, brown stopper
3. OrgansUnprocessed, frozen, 5 g, sealed plastic container
4. FeedPowdered, 5 g, sealed plastic container
5. WaterSealed plastic container

Pathology/Histopathology:

1. CarcassUnprocessed, as fresh as possible
2. Organs/parts of organsSterile, in petri dishes or plastic tubes, sealed
3. BiopsyFormaldehyde, 10 % solution
4. CrustFormaldehyde, 10 % solution
5. CytologySlides fixed in alcohol, 95 %

Serology:

1. Serum (clotted blood)Fresh, vacutainer, amount; 5 - 8 ml, brown stopper
2. Filter paperISOCODE filter strips

Avian Serology/Haematology:

1. Whole bloodCommercially available paediatric blood collecting tubes
2. Serum (clotted blood)Microtainer Serum Seperator tube
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